Standard Operating Procedures
Histopathology & Organ Pathology
Table of Contents
1.3 Animal and Tissue investigation request and information form
2.0 First line Phenotyping/Necropsy
2.4 Necropsy and organ list notes form
2.5 First Incision and Lymph Node Dissection
2.5.1 Diagram 1 - First Incision
2.5.2 Diagram 2 - Location of Lymph Nodes
2.5.3 Table 1 - Lymph Node Map
2.6.1 Diagram 3 - Mammary Tissue Location
2.7 Anatomy after Peritoneal Wall Incision
2.7.1 Diagram 4 - Peritoneal Wall Incision and Organs
2.8.1 Diagram 5 - Female and Male Urogenital Systems
2.9 Gastrointestinal Examination
2.9.1 Diagram 6 - Gastrointestinal Tract
2.10 Kidney and Adrenal Examination
2.10.1 Diagram 7 - Anatomy of the Mouse Kidney
2.10.2 Diagram 8 - Sternum Removal and Liver
2.11 Lungs and Thoracic Organs Examination
2.11.1 Diagram 9 - Lungs and Thoracic Organs
2.12.1 Diagram 10 - Heart and Principle Arteries and Veins
2.13.1 Diagram 11 - Mouse Brain Anatomy
2.16 Examination of Placentae, Feti and Neonates
2.17 Infection Control and Receipt of External Animals
4.0 Tissue Processing and Sectioning
6.0 Preliminary Histological Stains
6.2 Masson’s Trichrome (Modified)
7.0 Second Line Phenotyping/Targeted Histological Stains
7.1 Periodic Acid Schiff’s Reaction PAS for Glycogen
7.3 Verhoeff Van Gieson for Collagen and Elastic Fibres
7.4 Perl’s Prussian Blue Method
11.2 Zeiss digital slide scanner charge schedule
11.3 Digital slide scanner booking and priority schedule
11.4 Meta and Pascal charge schedule
11.5 Confocal booking and priority Schedule