Standard Operating Procedures
Histopathology & Organ Pathology
Table of Contents
1.3 Animal and Tissue investigation request and information form
2.0 First line Phenotyping/Necropsy
2.4 Necropsy and organ list notes form
2.5 First Incision and Lymph Node Dissection
2.5.1 Diagram 1 - First Incision
2.5.2 Diagram 2 - Location of Lymph Nodes
2.5.3 Table 1 - Lymph Node Map
2.6.1 Diagram 3 - Mammary Tissue Location
2.7 Anatomy after Peritoneal Wall Incision
2.7.1 Diagram 4 - Peritoneal Wall Incision and Organs
2.8.1 Diagram 5 - Female and Male Urogenital Systems
2.9 Gastrointestinal Examination
2.9.1 Diagram 6 - Gastrointestinal Tract
2.10 Kidney and Adrenal Examination
2.10.1 Diagram 7 - Anatomy of the Mouse Kidney
2.10.2 Diagram 8 - Sternum Removal and Liver
2.11 Lungs and Thoracic Organs Examination
2.11.1 Diagram 9 - Lungs and Thoracic Organs
2.12.1 Diagram 10 - Heart and Principle Arteries and Veins
2.13.1 Diagram 11 - Mouse Brain Anatomy
4.0 Tissue Processing and Sectioning
6.0 Preliminary Histological Stains
6.2 Masson’s Trichrome (Modified)
7.0 Second Line Phenotyping/Targeted Histological Stains
7.1 Periodic Acid Schiff’s Reaction PAS for Glycogen
7.3 Verhoeff Van Gieson for Collagen and Elastic Fibres
7.4 Perl’s Prussian Blue Method
9.1 Document 2- APN Histopathology and Organ Pathology Report
9.2 Document 3- Histology Observation Report
11.2 Zeiss digital slide scanner charge schedule
11.3 Digital slide scanner booking and priority schedule
11.4 Meta and Pascal charge schedule
11.5 Confocal booking and priority Schedule